SH2B1 antibody (AA 395-444)

Catalog No. ABIN6747173

The Rabbit Polyclonal anti-SH2B1 antibody has been validated for WB, IHC and IHC (p). It is suitable to detect SH2B1 in samples from Human, Mouse, Rat, Dog, Guinea Pig, Horse, Rabbit, Bat and Monkey.

Quick Overview for SH2B1 antibody (AA 395-444) (ABIN6747173)

Target: SH2B1 (SH2B Adaptor Protein 1 (SH2B1))

Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Horse, Rabbit, Bat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SH2B1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-SH2B1 Antibody

Binding Specificity: AA 395-444

Brand: IHC-plus™

Specificity: Human SH2B1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Horse, Guinea pig (100%) Bovine, Pig (92%) Rabbit (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa395-444 of human SH2B1 (Q9NRF2-2, NP_056318). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Galago, Marmoset, Mouse, Rat, Dog, Bat, Horse, Guinea pig (100%), Elephant, Panda, Bovine, Pig (92%), Rabbit, Opossum (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with 50 μL sterile ddH2O.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for SH2B1

Target: SH2B1 (SH2B Adaptor Protein 1 (SH2B1))

Alternative Name: SH2B1

Background: Name/Gene ID: SH2B1

Synonyms: SH2B1, KIAA1299, PSM, SH2B adapter protein 1, SH2-B signaling protein, SH2B, SH2B adaptor protein 1

Gene ID: 25970

NCBI Accession: NP_056318

UniProt: Q9NRF2