NASP antibody (AA 287-336)

Catalog No. ABIN6747182

The Rabbit Polyclonal anti-NASP antibody has been validated for WB, IHC and IHC (p). It is suitable to detect NASP in samples from Human, Rat, Mouse, Cow, Dog, Rabbit and Zebrafish (Danio rerio).

Quick Overview for NASP antibody (AA 287-336) (ABIN6747182)

Target: NASP (Nuclear Autoantigenic Sperm Protein (Histone-Binding) (NASP))

Reactivity: Human, Rat, Mouse, Cow, Dog, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NASP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-NASP Antibody

Binding Specificity: AA 287-336

Brand: IHC-plus™

Specificity: Human NASP

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Zebrafish (100%) Horse, Guinea pig (92%) Chicken (83%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa287-336 of human NASP (Q5T626, NP_689511). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Rabbit, Zebrafish (100%), Monkey, Bat, Horse, Opossum, Guinea pig (92%), Platypus (84%), Turkey, Chicken, Lizard (83%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for NASP

Target: NASP (Nuclear Autoantigenic Sperm Protein (Histone-Binding) (NASP))

Alternative Name: NASP

Background: Name/Gene ID: NASP

Synonyms: NASP, Histone H1-binding protein, PRO1999, FLB7527

Gene ID: 4678

NCBI Accession: NP_689511

UniProt: P49321