FTO antibody (N-Term)

Catalog No. ABIN769027

This Rabbit Polyclonal antibody specifically detects FTO in WB, IHC and IHC (p). It exhibits reactivity toward Human, Mouse, Rat, Cow, Sheep, Pig, Horse, Monkey and Hamster.

Quick Overview for FTO antibody (N-Term) (ABIN769027)

Target: FTO (Fat Mass and Obesity-Associated (FTO))

Reactivity: Human, Mouse, Rat, Cow, Sheep, Pig, Horse, Monkey, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FTO antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-FTO Antibody

Binding Specificity: N-Term

Specificity: Mouse FTO

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Sheep, Bovine, Horse, Pig (100%) Rabbit (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of mouse Fto (Q8BGW1, NP_036066). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Marmoset, Mouse, Rat, Sheep, Hamster, Panda, Bovine, Horse, Pig (100%), Elephant, Rabbit (92%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for FTO

Target: FTO (Fat Mass and Obesity-Associated (FTO))

Alternative Name: FTO

Background: Name/Gene ID: FTO

Synonyms: FTO, KIAA1752, Protein fto

Gene ID: 79068

NCBI Accession: NP_036066

UniProt: Q9C0B1