CPE antibody (AA 36-85)

Catalog No. ABIN6748532

This anti-CPE antibody is a Rabbit Polyclonal antibody detecting CPE in WB, IHC and IHC (p). Suitable for Human, Mouse, Rat, Cow, Guinea Pig, Rabbit, Zebrafish (Danio rerio), Bat, Monkey and Xenopus laevis.

Quick Overview for CPE antibody (AA 36-85) (ABIN6748532)

Target: CPE (Carboxypeptidase E (CPE))

Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Rabbit, Zebrafish (Danio rerio), Bat, Monkey, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CPE antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CPE Antibody

Binding Specificity: AA 36-85

Brand: IHC-plus™

Specificity: Human CPE

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Xenopus (100%) Pig (92%) Chicken, Zebrafish (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa36-85 of human CPE (P16870, NP_001864). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Bovine, Bat, Rabbit, Opossum, Guinea pig, Xenopus (100%), Pig (92%), Chicken, Stickleback, Zebrafish (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for CPE

Target: CPE (Carboxypeptidase E (CPE))

Alternative Name: Carboxypeptidase e / CPE

Background: Name/Gene ID: CPE
Subfamily: Metallopeptidase M14
Family: Exopeptidase

Synonyms: CPE, Carboxypeptidase E, CPH, Carboxypeptidase H, Enkephalin convertase

Gene ID: 1363

NCBI Accession: NP_001864

UniProt: P16870

Pathways: Peptide Hormone Metabolism, Synaptic Membrane