MCM3 antibody (AA 696-745)

Catalog No. ABIN6748568

The Rabbit Polyclonal anti-MCM3 antibody has been validated for WB, IHC and IHC (p). It is suitable to detect MCM3 in samples from Human, Monkey, Cow and Guinea Pig.

Quick Overview for MCM3 antibody (AA 696-745) (ABIN6748568)

Target: MCM3 (Minichromosome Maintenance Complex Component 3 (MCM3))

Reactivity: Human, Monkey, Cow, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MCM3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-MCM3 Antibody

Binding Specificity: AA 696-745

Brand: IHC-plus™

Specificity: Human MCM3

Predicted Reactivity: Percent identity by BLAST analysis: Human, Bovine (100%) Mouse, Rat, Horse (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa696-745 of human MCM3 (NP_002379). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Bovine, Guinea pig (100%), Galago, Mouse, Rat, Hamster, Horse (92%), Elephant, Bat (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB

Usage: ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for MCM3

Target: MCM3 (Minichromosome Maintenance Complex Component 3 (MCM3))

Alternative Name: MCM3

Background: Name/Gene ID: MCM3

Synonyms: MCM3, DNA replication factor MCM3, HCC5, HRlf beta subunit, p1-MCM3, RLF subunit beta, RLFB, p102, p1.h

Gene ID: 4172

NCBI Accession: NP_002379

UniProt: P25205

Pathways: DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA