ATF4 antibody (N-Term)

Catalog No. ABIN6748573

The Rabbit Polyclonal anti-ATF4 antibody has been validated for WB, IHC and IHC (p). It is suitable to detect ATF4 in samples from Human, Mouse, Rat, Dog, Cow, Monkey and Hamster.

Quick Overview for ATF4 antibody (N-Term) (ABIN6748573)

Target: ATF4 (Activating Transcription Factor 4 (Tax-Responsive Enhancer Element B67) (ATF4))

Reactivity: Human, Mouse, Rat, Dog, Cow, Monkey, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATF4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ATF4 Antibody

Binding Specificity: N-Term

Brand: IHC-plus™

Specificity: Mouse ATF4

Predicted Reactivity: Percent identity by BLAST analysis: Human, Rat, Dog (100%) Mouse, Sheep, Pig, Guinea pig (92%) Bovine (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of mouse Atf4 (Q5U4B2, NP_033846). Percent identity by BLAST analysis: Human, Gorilla, Monkey, Galago, Mouse, Rat, Hamster, Panda, Dog (100%), Gibbon, Sheep, Pig, Guinea pig (92%), Bovine, Opossum (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for ATF4

Target: ATF4 (Activating Transcription Factor 4 (Tax-Responsive Enhancer Element B67) (ATF4))

Alternative Name: ATF4

Background: Name/Gene ID: ATF4
Family: Transcription factor

Synonyms: ATF4, CREB-2, CREB2, TXREB, DNA-binding protein TAXREB67, TAXREB67

Gene ID: 468

NCBI Accession: NP_033846

UniProt: P18848

Pathways: Thyroid Hormone Synthesis, Myometrial Relaxation and Contraction, ER-Nucleus Signaling, Unfolded Protein Response