NFAT1 antibody (C-Term)

Catalog No. ABIN6748576

The Rabbit Polyclonal anti-NFAT1 antibody has been validated for WB, IHC and IHC (p). It is suitable to detect NFAT1 in samples from Human, Mouse, Rat, Dog, Horse, Guinea Pig, Rabbit, Pig, Monkey and Bat.

Quick Overview for NFAT1 antibody (C-Term) (ABIN6748576)

Target: NFAT1 (Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 2 (NFAT1))

Reactivity: Human, Mouse, Rat, Dog, Horse, Guinea Pig, Rabbit, Pig, Monkey, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFAT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-NFAT1 Antibody

Binding Specificity: C-Term

Brand: IHC-plus™

Specificity: Human NFATC2

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Rabbit, Horse, Pig, Guinea pig (100%) Bovine, Chicken (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human NFATC2 (Q13469, NP_775114). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Galago, Mouse, Rat, Elephant, Dog, Bat, Rabbit, Horse, Pig, Guinea pig (100%), Bovine, Opossum, Turkey, Zebra finch, Chicken, Platypus (92%), Xenopus (84%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for NFAT1

Target: NFAT1 (Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 2 (NFAT1))

Alternative Name: NFAT1 / NFATC2

Background: Name/Gene ID: NFATC2

Synonyms: NFATC2, NF-ATc2, NF-ATp, NFAT1, NFAT pre-existing subunit, NFATP

Gene ID: 4773

NCBI Accession: NP_775114

UniProt: Q13469

Pathways: RTK Signaling, WNT Signaling, Fc-epsilon Receptor Signaling Pathway, VEGF Signaling, BCR Signaling