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EIF4E antibody (C-Term)

The Rabbit Polyclonal anti-EIF4E antibody has been validated for WB, IHC and IHC (p). It is suitable to detect EIF4E in samples from Human, Rat, Mouse, Zebrafish (Danio rerio), Cow, Rabbit, Monkey, Dog, Guinea Pig, Horse, Chicken, Pig and Xenopus laevis.
Catalog No. ABIN6748583

Quick Overview for EIF4E antibody (C-Term) (ABIN6748583)

Target

See all EIF4E Antibodies
EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))

Reactivity

Human, Rat, Mouse, Zebrafish (Danio rerio), Cow, Rabbit, Monkey, Dog, Guinea Pig, Horse, Chicken, Pig, Xenopus laevis

Host

  • 108
  • 15
  • 2
Rabbit

Clonality

  • 98
  • 27
Polyclonal

Conjugate

  • 59
  • 9
  • 8
  • 6
  • 5
  • 4
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
This EIF4E antibody is un-conjugated

Application

  • 98
  • 52
  • 43
  • 28
  • 27
  • 26
  • 16
  • 16
  • 15
  • 14
  • 7
  • 6
  • 2
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • Binding Specificity

    • 40
    • 15
    • 7
    • 7
    • 6
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term

    Brand

    IHC-plus™

    Specificity

    Human EIF4E

    Predicted Reactivity

    Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Pig, Guinea pig, Chicken, Xenopus, Zebrafish (100%) Bovine, Rabbit (85%).

    Purification

    Immunoaffinity purified

    Immunogen

    Synthetic peptide from C-Terminus of human EIF4E (P06730, NP_001959). Percent identity by BLAST analysis: Human, Chimpanzee, Gibbon, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Armadillo, Xenopus, Frog, Smelt, Zebrafish (100%), Bat, Trout, Salmon (92%), Gorilla, Monkey (85%).

    Type of Immunogen: Synthetic peptide

    Isotype

    IgG
  • Application Notes

    Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

    Usage: ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

    Comment

    Target Species of Antibody: Human

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Distilled Water.

    Concentration

    Lot specific

    Buffer

    Lyophilized from PBS with 2 % sucrose

    Handling Advice

    Avoid repeat freeze-thaw cycles.

    Storage

    4 °C,-20 °C

    Storage Comment

    Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
    Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
  • Target

    EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))

    Alternative Name

    EIF4E

    Background

    Name/Gene ID: EIF4E

    Synonyms: EIF4E, EIF-4E, EIF4E1, EIF4EL1, EIF4F, CBP, EIF-4F 25 kDa subunit, MRNA cap-binding protein

    Gene ID

    1977

    NCBI Accession

    NP_001959

    UniProt

    P06730

    Pathways

    BCR Signaling
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