EIF4E antibody (C-Term)
Quick Overview for EIF4E antibody (C-Term) (ABIN6748583)
Target
See all EIF4E AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Binding Specificity
- C-Term
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Brand
- IHC-plus™
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Specificity
- Human EIF4E
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Predicted Reactivity
- Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Pig, Guinea pig, Chicken, Xenopus, Zebrafish (100%) Bovine, Rabbit (85%).
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Purification
- Immunoaffinity purified
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Immunogen
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Synthetic peptide from C-Terminus of human EIF4E (P06730, NP_001959). Percent identity by BLAST analysis: Human, Chimpanzee, Gibbon, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Armadillo, Xenopus, Frog, Smelt, Zebrafish (100%), Bat, Trout, Salmon (92%), Gorilla, Monkey (85%).
Type of Immunogen: Synthetic peptide -
Isotype
- IgG
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Application Notes
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Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)
Usage: ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. -
Comment
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Target Species of Antibody: Human
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Distilled Water.
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Concentration
- Lot specific
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Buffer
- Lyophilized from PBS with 2 % sucrose
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Handling Advice
- Avoid repeat freeze-thaw cycles.
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Storage
- 4 °C,-20 °C
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Storage Comment
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Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
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- EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))
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Alternative Name
- EIF4E
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Background
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Name/Gene ID: EIF4E
Synonyms: EIF4E, EIF-4E, EIF4E1, EIF4EL1, EIF4F, CBP, EIF-4F 25 kDa subunit, MRNA cap-binding protein -
Gene ID
- 1977
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NCBI Accession
- NP_001959
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UniProt
- P06730
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Pathways
- BCR Signaling
Target
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