EIF4E antibody (C-Term)

Catalog No. ABIN6748583

The Rabbit Polyclonal anti-EIF4E antibody is suitable to detect EIF4E in samples from Human, Rat, Mouse, Zebrafish (Danio rerio), Cow, Monkey, Rabbit, Dog, Guinea Pig, Horse, Chicken, Pig and Xenopus laevis. It has been validated for WB, IHC and IHC (p).

Quick Overview for EIF4E antibody (C-Term) (ABIN6748583)

Target: EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))

Reactivity: Human, Rat, Mouse, Zebrafish (Danio rerio), Cow, Monkey, Rabbit, Dog, Guinea Pig, Horse, Chicken, Pig, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF4E antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-EIF4E Antibody

Binding Specificity: C-Term

Specificity: Human EIF4E

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Pig, Guinea pig, Chicken, Xenopus, Zebrafish (100%) Bovine, Rabbit (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human EIF4E (P06730, NP_001959). Percent identity by BLAST analysis: Human, Chimpanzee, Gibbon, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Armadillo, Xenopus, Frog, Smelt, Zebrafish (100%), Bat, Trout, Salmon (92%), Gorilla, Monkey (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for EIF4E

Target: EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))

Alternative Name: EIF4E

Background: Name/Gene ID: EIF4E

Synonyms: EIF4E, EIF-4E, EIF4E1, EIF4EL1, EIF4F, CBP, EIF-4F 25 kDa subunit, MRNA cap-binding protein

Gene ID: 1977

NCBI Accession: NP_001959

UniProt: P06730

Pathways: BCR Signaling