RHOT1 antibody (N-Term)

Catalog No. ABIN6749728

The Rabbit Polyclonal anti-RHOT1 antibody has been validated for WB, IHC and IHC (p). It is suitable to detect RHOT1 in samples from Human, Mouse, Rat, Cow, Guinea Pig, Horse, Rabbit, Bat, Chicken, Monkey and Pig.

Quick Overview for RHOT1 antibody (N-Term) (ABIN6749728)

Target: RHOT1 (Ras Homolog Gene Family, Member T1 (RHOT1))

Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Horse, Rabbit, Bat, Chicken, Monkey, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RHOT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-RHOT1 Antibody

Binding Specificity: N-Term

Brand: IHC-plus™

Specificity: Human RHOT1

Predicted Reactivity: Percent identity by BLAST analysis: Rat, Rabbit, Horse, Pig, Guinea pig, Chicken (100%) Xenopus (92%) Dog (82%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human RHOT1 (Q8IXI2, NP_060777). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Seabass, Stickleback (100%), Panda, Dog, Xenopus, Zebrafish, Drosophila (92%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 - 10 μg/mL), WB

Usage: ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 0.25 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for RHOT1

Target: RHOT1 (Ras Homolog Gene Family, Member T1 (RHOT1))

Alternative Name: RHOT1

Background: Name/Gene ID: RHOT1
Family: Ras GTPase superfamily IPR001806

Synonyms: RHOT1, ARHT1, HMiro-1, MIRO-1, Mitochondrial Rho GTPase 1, MIRO1, Mitochondrial Rho 1, Ras homolog family member T1

Gene ID: 55288

NCBI Accession: NP_060777

UniProt: Q8IXI2