PODXL antibody (AA 451-558)

Catalog No. ABIN675264

This anti-PODXL antibody is a Rabbit Polyclonal antibody detecting PODXL in WB, ELISA, FACS, IHC (p), IHC (fro), IF (p) and IF (cc). Suitable for Human, Rat and Mouse. This Primary Antibody has been cited in 1 publication.

Quick Overview for PODXL antibody (AA 451-558) (ABIN675264)

Target: PODXL (Podocalyxin-Like (PODXL))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PODXL antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Product Details anti-PODXL Antibody

Binding Specificity: AA 451-558

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Podocalyxin

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-PODXL antibody (ABIN675264)

Xing, Ye, Chen, Hu, Chen: "Hydrochloride pioglitazone protects diabetic rats against podocyte injury through preserving glomerular podocalyxin expression." in: Arquivos brasileiros de endocrinologia e metabologia, Vol. 58, Issue 6, pp. 630-9, (2014) (PubMed).

Target Details for PODXL

Target: PODXL (Podocalyxin-Like (PODXL))

Alternative Name: Podocalyxin

Background:

Synonyms: PC, PCLP, Gp2, PCLP-1, Podocalyxin, GCTM-2 antigen, Podocalyxin-like protein 1, PODXL, PCLP1

Background: Involved in the regulation of both adhesion and cell morphology and cancer progression. Function as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up inital epithelial polarization and the apical lumen formation during renal tubulogenesis. Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. Affects EZR-dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells.

Gene ID: 5420

UniProt: O00592

Pathways: Tube Formation