SMPD1 antibody (AA 396-445)

Catalog No. ABIN6752980

This Rabbit Polyclonal antibody specifically detects SMPD1 in WB, IHC and IHC (p). It exhibits reactivity toward Human, Mouse, Rat, Cow, Sheep, Dog, Horse, Rabbit, Pig, Bat and Monkey.

Quick Overview for SMPD1 antibody (AA 396-445) (ABIN6752980)

Target: SMPD1 (Sphingomyelin phosphodiesterase 1, Acid Lysosomal (SMPD1))

Reactivity: Human, Mouse, Rat, Cow, Sheep, Dog, Horse, Rabbit, Pig, Bat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMPD1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-SMPD1 Antibody

Binding Specificity: AA 396-445

Specificity: Human SMPD1

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa396-445 of human SMPD1 (P17405, NP_000534). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Sheep, Dog, Bovine, Bat, Rabbit, Horse, Pig (100%), Guinea pig (92%), Mouse, Rat, Elephant (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for SMPD1

Target: SMPD1 (Sphingomyelin phosphodiesterase 1, Acid Lysosomal (SMPD1))

Alternative Name: SMPD1 / Acid Sphingomyelinase

Background: Name/Gene ID: SMPD1
Family: Phosphodiesterase - Other

Synonyms: SMPD1, Acid sphingomyelinase, ASM, NPD, ASMASE

Gene ID: 6609

NCBI Accession: NP_000534