Splicing Factor 1 antibody (AA 65-114)

Catalog No. ABIN6754883

The Rabbit Polyclonal anti-Splicing Factor 1 antibody has been validated for WB, IHC and IHC (p). It is suitable to detect Splicing Factor 1 in samples from Human, Mouse, Rat, Dog, Rabbit, Cow, Horse, Guinea Pig, Zebrafish (Danio rerio), Monkey, Pig, Bat and Xenopus laevis.

Quick Overview for Splicing Factor 1 antibody (AA 65-114) (ABIN6754883)

Target: Splicing Factor 1 (SF1)

Reactivity: Human, Mouse, Rat, Dog, Rabbit, Cow, Horse, Guinea Pig, Zebrafish (Danio rerio), Monkey, Pig, Bat, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Splicing Factor 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Splicing Factor 1 Antibody

Binding Specificity: AA 65-114

Brand: IHC-plus™

Specificity: Human SF1

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa65-114 of human SF1 (Q15637, NP_004621). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Baboon, Monkey, Galago, Marmoset, Mouse, Rat, Shrew, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig, Armadillo, Platypus, Xenopus, Stickleback, Zebrafish (100%), Drosophila (84%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB (1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for Splicing Factor 1

Target: Splicing Factor 1 (SF1)

Alternative Name: SF1

Background: Name/Gene ID: SF1

Synonyms: SF1, D11S636, MBBP, ZCCHC25, Transcription factor ZFM1, Zinc finger gene in MEN1 locus, Zinc finger protein 162, BBP, ZNF162, Splicing factor 1, ZFM1

Gene ID: 7536

NCBI Accession: NP_004621

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Maintenance of Protein Location