GAPDH antibody (AA 38-87)

Catalog No. ABIN6754891

The Rabbit Polyclonal anti-GAPDH antibody has been validated for WB, IHC and IHC (p). It is suitable to detect GAPDH in samples from Human, Rabbit, Pig, Monkey, Dog, Sheep, Horse and Guinea Pig.

Quick Overview for GAPDH antibody (AA 38-87) (ABIN6754891)

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Reactivity: Human, Rabbit, Pig, Monkey, Dog, Sheep, Horse, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GAPDH antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-GAPDH Antibody

Binding Specificity: AA 38-87

Brand: IHC-plus™

Specificity: Human GAPDH

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa38-87 of human GAPDH (Q2TSD0, NP_002037). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Ferret, Sheep, Elephant, Panda, Dog, Cat, Rabbit, Horse, Pig, Guinea pig, Platypus (100%), Mouse, Rat, Hamster, Bovine, Opossum, Armadillo (92%), Nematode (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB (0.2 - 1 μg/mL)

Usage: ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for GAPDH

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Alternative Name: GAPDH

Background: Name/Gene ID: GAPDH

Synonyms: GAPDH, A1 40 kd subunit, Activator 1 40 kd subunit, G3PD, GAPD, G3pdh, Rfc40, Rf-c 40 kd subunit

Gene ID: 2597

NCBI Accession: NP_002037