H2AFY antibody (AA 144-193)

Catalog No. ABIN6754907

The Rabbit Polyclonal anti-H2AFY antibody has been validated for WB, IHC and IHC (p). It is suitable to detect H2AFY in samples from Human, Rat, Mouse, Cow, Horse and Monkey.

Quick Overview for H2AFY antibody (AA 144-193) (ABIN6754907)

Target: H2AFY (H2A Histone Family, Member Y (H2AFY))

Reactivity: Human, Rat, Mouse, Cow, Horse, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This H2AFY antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-H2AFY Antibody

Binding Specificity: AA 144-193

Brand: IHC-plus™

Specificity: Human H2AFY

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa144-193 of human H2AFY (O75367, NP_001035248). Percent identity by BLAST analysis: Human, Chimpanzee, Monkey, Marmoset, Mouse, Rat, Bovine, Horse, Platypus (100%), Gorilla, Gibbon, Galago, Elephant, Panda, Dog, Rabbit, Pig, Guinea pig, Turkey, Zebra finch, Chicken (93%), Opossum (86%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for H2AFY

Target: H2AFY (H2A Histone Family, Member Y (H2AFY))

Alternative Name: H2AFY / MACROH2A1

Background: Name/Gene ID: H2AFY

Synonyms: H2AFY, Core histone macro-H2A.1, Histone macroH2A1.1, H2A histone family, member Y, H2A.y, Histone H2A.y, MACROH2A1.1, MacroH2A1.2, H2AF12M, Histone macroH2A1.2, MACROH2A1, Histone macroH2A1, MH2A1

Gene ID: 9555

NCBI Accession: NP_001035248