T Brachyury Protein (AA 251-300) antibody

Catalog No. ABIN6755269

This Rabbit Polyclonal antibody specifically detects in WB, IHC and IHC (p). It exhibits reactivity toward Human, Cow, Dog, Rat, Horse and Pig.

Quick Overview for T Brachyury Protein (AA 251-300) antibody (ABIN6755269)

Target: T Brachyury Protein

Reactivity: Human, Cow, Dog, Rat, Horse, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details

Binding Specificity: AA 251-300

Brand: IHC-plus™

Specificity: Human T / Brachyury

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa251-300 of human T (O15178, NP_003172). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Rat, Panda, Dog, Horse (100%), Marmoset, Mouse, Platypus, Xenopus, Newt (92%), Guinea pig (91%), Opossum (85%), Elephant (84%), Bovine, Pig, Zebra finch (83%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details

Target: T Brachyury Protein

Alternative Name: T / Brachyury

Background: Name/Gene ID: T / Brachyury

Synonyms: T / Brachyury, Brachyury, Brachyury protein, Protein T, TFT, T, T brachyury (mouse) homolog, T brachyury homolog, T, brachyury homolog (mouse)

Gene ID: 6862

NCBI Accession: NP_003172

Pathways: Tube Formation