P4HB antibody (AA 101-150)

Catalog No. ABIN6755442

This anti-P4HB antibody is a Rabbit Polyclonal antibody detecting P4HB in WB, IHC and IHC (p). Suitable for Human, Mouse, Rat, Hamster, Cow, Dog, Monkey, Rabbit, Horse and Chicken.

Quick Overview for P4HB antibody (AA 101-150) (ABIN6755442)

Target: P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))

Reactivity: Human, Mouse, Rat, Hamster, Cow, Dog, Monkey, Rabbit, Horse, Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This P4HB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-P4HB Antibody

Binding Specificity: AA 101-150

Brand: IHC-plus™

Specificity: Human P4HB / PDI

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa101-150 of human P4HB (P07237, NP_000909). Percent identity by BLAST analysis: Human, Chimpanzee, Orangutan, Gibbon, Monkey, Mouse, Rat, Hamster, Bovine, Rabbit, Horse, Chicken, Platypus (100%), Elephant, Opossum, Guinea pig (92%), Gorilla (87%), Galago, Dog, Zebra finch, Xenopus (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB

Usage: ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 0.5 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for P4HB

Target: P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))

Alternative Name: PDI / P4HB

Background: Name/Gene ID: P4HB

Synonyms: P4HB, ERBA2L, DSI, GIT, p4HB, p4Hbeta, PDIA1, PHDB, PO4DB, PROHB, Protocollagen hydroxylase, Protein disulfide-isomerase, PO4HB, PDI

Gene ID: 5034

NCBI Accession: NP_000909

Pathways: Maintenance of Protein Location, Cell RedoxHomeostasis, Lipid Metabolism