ADA antibody (AA 288-337)

Catalog No. ABIN6755450

The Rabbit Polyclonal anti-ADA antibody has been validated for WB, IHC and IHC (p). It is suitable to detect ADA in samples from Human, Mouse, Cow, Guinea Pig, Rabbit, Hamster, Monkey and Bat.

Quick Overview for ADA antibody (AA 288-337) (ABIN6755450)

Target: ADA (Adenosine Deaminase (ADA))

Reactivity: Human, Mouse, Cow, Guinea Pig, Rabbit, Hamster, Monkey, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ADA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ADA Antibody

Binding Specificity: AA 288-337

Brand: IHC-plus™

Specificity: Human ADA

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa288-337 of human ADA (P00813, NP_000013). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Hamster, Elephant, Bovine, Bat, Rabbit, Guinea pig (100%), Rat, Dog, Horse (92%), Zebra finch, Xenopus (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 - 10 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for ADA

Target: ADA (Adenosine Deaminase (ADA))

Alternative Name: ADA / Adenosine Deaminase

Background: Name/Gene ID: ADA

Synonyms: ADA, Adenosine deaminase, ADA1, Adenosine aminohydrolase

Gene ID: 100

NCBI Accession: NP_000013

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Ribonucleoside Biosynthetic Process