EGLN3 antibody (C-Term)

Catalog No. ABIN6755451

The Rabbit Polyclonal anti-EGLN3 antibody (ABIN6755451) specifically detects EGLN3 in WB, IHC and IHC (p). The antibody is reactive with Human, Mouse, Rat, Cow, Dog, Horse, Pig, Rabbit, Monkey, Guinea Pig and Chicken samples.

Quick Overview for EGLN3 antibody (C-Term) (ABIN6755451)

Target: EGLN3 (Egl-9 Family Hypoxia Inducible Factor 3 (EGLN3))

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Pig, Rabbit, Monkey, Guinea Pig, Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EGLN3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-EGLN3 Antibody

Binding Specificity: C-Term

Specificity: Human EGLN3 / PHD3

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human EGLN3 (Q9H6Z9, NP_071356). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus (100%), Bat (92%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB (0.2 - 1 μg/mL)

Usage: ELISA titer using peptide based assay: 1:1562500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for EGLN3

Target: EGLN3 (Egl-9 Family Hypoxia Inducible Factor 3 (EGLN3))

Alternative Name: EGLN3 / PHD3

Background: Name/Gene ID: EGLN3

Synonyms: EGLN3, HPH-1, HIF prolyl hydroxylase 3, HIF-prolyl hydroxylase 3, HIFPH3, PHD3, EGL nine (C.elegans) homolog 3, Egl nine homolog 3, HIF-PH3, HIFP4H3

Gene ID: 112399

NCBI Accession: NP_071356

Pathways: Positive Regulation of Endopeptidase Activity