PDE4B antibody (AA 467-516)

Catalog No. ABIN6755460

The Rabbit Polyclonal anti-PDE4B antibody is suitable to detect PDE4B in samples from Human, Rat, Cow, Dog, Guinea Pig, Horse, Pig, Bat and Monkey. It has been validated for WB, IHC and IHC (p).

Quick Overview for PDE4B antibody (AA 467-516) (ABIN6755460)

Target: PDE4B (phosphodiesterase 4B, cAMP-Specific (PDE4B))

Reactivity: Human, Rat, Cow, Dog, Guinea Pig, Horse, Pig, Bat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDE4B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PDE4B Antibody

Binding Specificity: AA 467-516

Specificity: Human PDE4B

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa467-516 of human PDE4B (Q5TEK5, NP_001032416). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Rat, Dog, Bovine, Bat, Horse, Pig (100%), Galago, Elephant, Rabbit, Opossum (92%), Turkey, Zebra finch, Chicken (90%), Mouse (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for PDE4B

Target: PDE4B (phosphodiesterase 4B, cAMP-Specific (PDE4B))

Alternative Name: PDE4B

Background: Name/Gene ID: PDE4B
Subfamily: PDE - cAMP-specific
Family: Phosphodiesterase

Synonyms: PDE4B, Pde-ivb, PDE32, PDE4B5, PDEIVB, Phosphodiesterase 4b, DPDE4, PDE4B3

Gene ID: 5142

NCBI Accession: NP_001032416

Pathways: Cellular Response to Molecule of Bacterial Origin, cAMP Metabolic Process, Myometrial Relaxation and Contraction