AKR1C1 antibody (AA 50-99)

Catalog No. ABIN6755520

This Rabbit Polyclonal antibody specifically detects AKR1C1 in WB, IHC and IHC (p). It exhibits reactivity toward Human and Monkey.

Quick Overview for AKR1C1 antibody (AA 50-99) (ABIN6755520)

Target: AKR1C1 (DDH) (Dihydrodiol Dehydrogenase (DDH))

Reactivity: Human, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AKR1C1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-AKR1C1 Antibody

Binding Specificity: AA 50-99

Brand: IHC-plus™

Specificity: Human AKR1C1

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa50-99 of human AKR1C1 (Q04828, NP_001344). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset (100%), Mouse, Rat, Dog, Bovine, Rabbit, Pig, Opossum, Guinea pig, Catfish, Salmon, Smelt (92%), Horse, Xenopus, Frog (85%), Bat, Platypus, Sablefish, Pike, Drosophila, Beetle (84%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with 50 μL sterile ddH2O.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for AKR1C1

Target: AKR1C1 (DDH) (Dihydrodiol Dehydrogenase (DDH))

Alternative Name: DDH / AKR1C1

Background: Name/Gene ID: AKR1C1

Synonyms: AKR1C1, 2-ALPHA-HSD, 20-alpha-HSD, Aldo-keto reductase C, Bile acid binding protein, Chlordecone reductase homolog, DDH1, Dihydrodiol dehydrogenase 2, DD1, DDH, Dihydrodiol dehydrogenase 1/2, DD1/DD2, Dihydrodiol dehydrogenase 1, H-37, HAKRC, HBAB, Indanol dehydrogenase, MBAB

Gene ID: 1645

NCBI Accession: NP_001344

Pathways: Steroid Hormone Biosynthesis, C21-Steroid Hormone Metabolic Process