PFN1 antibody (N-Term)

Catalog No. ABIN6756639

The Rabbit Polyclonal anti-PFN1 antibody has been validated for WB, IHC and IHC (p). It is suitable to detect PFN1 in samples from Human, Mouse, Rat, Monkey, Cow, Guinea Pig, Horse and Pig.

Quick Overview for PFN1 antibody (N-Term) (ABIN6756639)

Target: PFN1 (Profilin 1 (PFN1))

Reactivity: Human, Mouse, Rat, Monkey, Cow, Guinea Pig, Horse, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PFN1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PFN1 Antibody

Binding Specificity: N-Term

Brand: IHC-plus™

Specificity: Human PFN1 / Profilin 1

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human PFN1 (P07737, NP_005013). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Bovine, Horse, Pig, Opossum, Guinea pig (100%), Bat (92%), Catfish, Zebrafish (90%), Xenopus (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB (0.2 - 1 μg/mL)

Usage: ELISA titer using peptide based assay: 1:12500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for PFN1

Target: PFN1 (Profilin 1 (PFN1))

Alternative Name: PFN1 / Profilin 1

Background: Name/Gene ID: PFN1

Synonyms: PFN1, ALS18, Profilin-1, Profilin 1, Profilin I

Gene ID: 5216

NCBI Accession: NP_005013

Pathways: Regulation of Actin Filament Polymerization, Tube Formation, Maintenance of Protein Location