PEPD antibody (AA 359-408)

Catalog No. ABIN6756656

The Rabbit Polyclonal anti-PEPD antibody has been validated for WB, IHC and IHC (p). It is suitable to detect PEPD in samples from Human, Mouse, Rat, Dog, Guinea Pig, Horse, Pig and Bat. There is 1 publication available.

Quick Overview for PEPD antibody (AA 359-408) (ABIN6756656)

Target: PEPD (Peptidase D (PEPD))

Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Horse, Pig, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PEPD antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PEPD Antibody

Binding Specificity: AA 359-408

Brand: IHC-plus™

Specificity: Human PEPD

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa359-408 of human PEPD (P12955, NP_000276). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Marmoset, Mouse, Rat, Dog, Bat, Horse, Pig, Opossum, Guinea pig, Stickleback (100%), Monkey, Galago, Rabbit, Turkey, Chicken (92%), Bovine (91%), Zebrafish (85%), Elephant (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

References for anti-PEPD antibody (ABIN6756656)

Rešetar Maslov, Farkaš, Rubić, Kuleš, Beletić, Beer Ljubić, Šmit, Mrljak, Torti: "Serum Proteomic Profiles Reflect the Stages of Myxomatous Mitral Valve Disease in Dogs." in: International journal of molecular sciences, Vol. 24, Issue 8, (2023) (PubMed).

Target Details for PEPD

Target: PEPD (Peptidase D (PEPD))

Alternative Name: PEPD / PROLIDASE

Background: Name/Gene ID: PEPD
Subfamily: Metallopeptidase M24B
Family: Exopeptidase

Synonyms: PEPD, Aminoacyl-L-proline hydrolase, Imidodipeptidase, PRD, PROLIDASE, X-Pro dipeptidase, Xaa-Pro dipeptidase, Peptidase D, Proline dipeptidase

Gene ID: 5184

NCBI Accession: NP_000276