TPSAB1 antibody (AA 29-273)
Quick Overview for TPSAB1 antibody (AA 29-273) (ABIN6757092)
Target
See all TPSAB1 AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Binding Specificity
- AA 29-273
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Specificity
- Recognizes murine Tryptase, beta1 in direct ELISAs and Western Blots. Shows ~10 % cross-reactivity with recombinant human TPSB2, 5 % cross-reactivity with recombinant human TPS1 and less than 1 % cross-reactivity with recombinant human TPSG1 and recombinant murine MCPT1.
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Purification
- Immunoaffinity purified
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Immunogen
- NS0-derived, recombinant murine Tryptase beta1 (aa29-273).
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Isotype
- IgG
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Application Notes
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Approved: ELISA (0.5 - 1 μg/mL), IF, IP, WB (0.1 - 0.2 μg/mL)
Usage: Suitable for use in ELISA, Western Blot and Immunoprecipitation. Direct ELISA: 0.5-1 μg/mL. The detection limit for recombinant murine Tryptase beta1 is ~0.5 ng/well. Western Blot: 0.1-0.2 μg/mL. The detection limit for recombinant murine Tryptase beta1 is ~1 ng/lane under non-reducing and reducing conditions. Immunoprecipitation: Immunoprecipitates recombinant, murine Tryptase beta1 from conditioned media of transfected NS0 cells. The recovered recombinant murine Tryptase beta1 can be detected by Western Blot analysis using a monoclonal antibody. -
Comment
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Target Species of Antibody: Mouse
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- Lot specific
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Buffer
- PBS, pH 7.4, 5 % trehalose.
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Handling Advice
- Avoid repeat freeze-thaw cycles.
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Storage
- 4 °C,-20 °C
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Storage Comment
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- TPSAB1 (Tryptase alpha/beta 1 (TPSAB1))
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Alternative Name
- TPSAB1 / Mast Cell Tryptase
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Background
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Name/Gene ID: TPSAB1
Subfamily: Serine S1
Family: Protease
Synonyms: TPSAB1, Alpha tryptase, Mast cell alpha II tryptase, Tryptase alpha-1, Tryptase beta I, Tryptase-III, TPSB1, Tryptase alpha II, TPS1, TPS2, Tryptase beta-1, Tryptase-1, Tryptase-I, Mast cell beta I tryptase, Tryptase alpha/beta 1, Tryptase alpha/beta-1, Tryptase beta 1, Tryptase I -
Gene ID
- 7177
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UniProt
- Q15661
Target
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