GRIA4 antibody (AA 889-902)

Catalog No. ABIN6757120

This anti-GRIA4 antibody is a Rabbit Polyclonal antibody detecting GRIA4 in WB, IHC and IHC (fro). Suitable for Mouse and Rat.

Quick Overview for GRIA4 antibody (AA 889-902) (ABIN6757120)

Target: GRIA4 (Glutamate Receptor, Ionotropic, AMPA 4 (GRIA4))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRIA4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-GRIA4 Antibody

Binding Specificity: AA 889-902

Specificity: Western blot of rat brain shows a single band co-migrating with GluR4 expressed in transfected cells. No cross-reaction with GluR1-3. The antibody appears to recognize GluR4 in several other species, but has not been extensively characterized in these cases. Species Reactivity: rat and mouse.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Opossum, Turkey, Chicken, Platypus, Lizard, Xenopus, Trachemys (100%).

Purification: Affinity purified

Immunogen: Carboxy terminus peptide of rat GluR4 conjugated to BSA with glutaraldehyde (RQSSGLAVIASDLP). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Opossum, Turkey, Chicken, Platypus, Lizard, Xenopus, Trachemys (100%).

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-Fr (1:10 - 1:50), WB (1 - 3 μg/mL)

Usage: Can be used for immunocytochemistry using paraformaldehyde or paraformaldehyde/glutaraldehyde fixed tissue with light and electron microscopy. Cryostat and vibratome sections can be used with or without Triton X-100 treatment. Final concentrations used were 1-3 μg/mL. Western blot analysis can be done at final concentrations of 0.5-2.5 μg/mL.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: deionized water

Concentration: Lot specific

Buffer: PBS, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for GRIA4

Target: GRIA4 (Glutamate Receptor, Ionotropic, AMPA 4 (GRIA4))

Alternative Name: GRIA4 / GLUR4

Background: Name/Gene ID: GRIA4
Subfamily: Glutamate receptor - ionotropic (NMDA receptor)
Family: Ion Channel

Synonyms: GRIA4, GluA4, GluR-4, GLUR4, GLUR4C, Glutamate receptor type d, GluR-D, GLURD, Glutamate receptor 4

Gene ID: 2893

UniProt: P48058

Pathways: PI3K-Akt Signaling