WNK3 antibody (AA 451-550) (Biotin)

Catalog No. ABIN675715

This Rabbit Polyclonal antibody specifically detects WNK3 in WB, ELISA, IHC (p) and IHC (fro). It exhibits reactivity toward Human and Rat.

Quick Overview for WNK3 antibody (AA 451-550) (Biotin) (ABIN675715)

Target: WNK3 (WNK Lysine Deficient Protein Kinase 3 (WNK3))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WNK3 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-WNK3 Antibody (Biotin)

Binding Specificity: AA 451-550

Cross-Reactivity: Human, Rat

Predicted Reactivity: Mouse,Cow,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human WNK3 protein

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for WNK3

Target: WNK3 (WNK Lysine Deficient Protein Kinase 3 (WNK3))

Alternative Name: WNK3 protein

Background:

Synonyms: PRKWNK3, Serine/threonine-protein kinase WNK3, Protein kinase lysine-deficient 3, Protein kinase with no lysine 3, WNK3, KIAA1566

Background: Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Phosphorylates WNK4. Regulates the phosphorylation of SLC12A1 and SLC12A2. Increases Ca(2+) influx mediated by TRPV5 and TRPV6 by enhancing their membrane expression level via a kinase-dependent pathway. Inhibits the activity of KCNJ1 by decreasing its expression at the cell membrane in a non-catalytic manner. Isoform 1, isoform 2, isoform 3 and isoform 4 stimulate the activity of SLC12A1, SLC12A2 and SLC12A3 and inhibit the activity of SLC12A4, SLC12A5, SLC12A6 and SLC12A7. According to PubMed:19470686, isoform 1 inhibits the activity of SLC12A3.

Gene ID: 65267

UniProt: Q9BYP7