CLDN14 antibody (Middle Region)

Catalog No. ABIN6757213

The Rabbit Polyclonal anti-CLDN14 antibody (ABIN6757213) specifically detects CLDN14 in WB, IF and ICC. The antibody is reactive with Human samples.

Quick Overview for CLDN14 antibody (Middle Region) (ABIN6757213)

Target: CLDN14 (Claudin 14 (CLDN14))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CLDN14 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-CLDN14 Antibody

Binding Specificity: Middle Region

Specificity: Reacts with the ~22kD human Claudin-14.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide derived from the mid region of the human Claudin-14 protein.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: ICC, IF (3 - 9 μg/mL), WB (1 - 3 μg/mL)

Usage: Suitable for use in Immunocytochemistry, Immunofluorescence and Western Blot. Immunofluorescence: 3-9 μg/mL, Immunofluorescence assays were performed using human Claudin 14 transfected-MDCK cells that were fixed with 1 % paraformaldehyde, permeabilized with 0.2 % Triton X-100 and quenched with 50 mM ammonium chloride. Western Blot: 1-3 μg/mL, Claudin-14 oligomers are observed at above 40kD in Western Blot using HepG2 cell lysates. Positive control: WB: Human HepG2 hepatocellular carcinoma cell lysate.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide, 40 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for CLDN14

Target: CLDN14 (Claudin 14 (CLDN14))

Alternative Name: CLDN14 / Claudin 14

Background: Name/Gene ID: CLDN14
Family: Claudin

Synonyms: CLDN14, Claudin 14, DFNB29, Claudin-14

Gene ID: 23562

UniProt: O95500

Pathways: Cell-Cell Junction Organization, Hepatitis C