LDLR antibody (AA 184-196)

Catalog No. ABIN233315

The Rabbit Polyclonal anti-LDLR antibody has been validated for WB, IHC, ELISA, FACS and IHC (fro). It is suitable to detect LDLR in samples from Human and Rat.

Quick Overview for LDLR antibody (AA 184-196) (ABIN233315)

Target: LDLR (Low Density Lipoprotein Receptor (LDLR))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LDLR antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-LDLR Antibody

Binding Specificity: AA 184-196

Specificity: Recognizes human LDLR. Does not inhibit binding of LDL. In Western Blot, recognizes the 160kD of LDLR and a 120kD band of the LDLR precursor from fibroblasts, hepatocytes and monocytic cells cultured in the presence of lipoprotein-deficient serum. Sequence not conserved in VLVL Receptor and LRP. Species cross-reactivity: rat. Species sequence Homology: mouse and bovine.

Purification: Protein A purified

Immunogen: Synthetic peptide of the complement homology region within the ligand binding site of the LDL receptor extracellular domain. Epitope between aa184-196, the linker region between repeats 4 and 5.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, Flo (1:5), IHC, IHC-Fr (1:10), WB (1:50 - 1:100)

Usage: Suitable for use in ELISA, Western Blot, FACS and Immunohistochemistry. Western Blot: 1:50-1:100 using ECL (non-reducing). FACS: 1:5. Immunohistochemistry: 1:10. Note: It is best to work with partially purified material. In the case of rat (probably also mouse), induce LDLR expression prior to the isolation of culture cells or tissue homogenate. It is always better to use cultured cells. In rat, use estradiol to induce LDLR expression. Positive control: HepG2 cells and liver sections.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.5 % BSA.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for LDLR

Target: LDLR (Low Density Lipoprotein Receptor (LDLR))

Alternative Name: LDLR / LDL Receptor

Background: Name/Gene ID: LDLR

Synonyms: LDLR, FHC, LDL receptor, LDLCQ2

Gene ID: 3949

UniProt: P01130

Pathways: Hepatitis C, Lipid Metabolism