CD33 antibody (AA 261-364)

Catalog No. ABIN676958

The Rabbit Polyclonal anti-CD33 antibody has been validated for FACS, WB, ELISA, IHC (fro), IF (p), IF (cc) and IHC (p). It is suitable to detect CD33 in samples from Human. There are 2+ publications available.

Quick Overview for CD33 antibody (AA 261-364) (ABIN676958)

Target: CD33

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD33 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CD33 Antibody

Binding Specificity: AA 261-364

Cross-Reactivity: Human

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human CD33

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-CD33 antibody (ABIN676958)

Shi, Jiang, Li, Shan, Li, An, Yang, Xu: "Sodium selenite alters microtubule assembly and induces apoptosis in vitro and in vivo." in: Journal of hematology & oncology, Vol. 6, Issue 1, pp. 7, (2013) (PubMed).

An, Shi, Wei, Hua, Ci, Jiang, Li, Wu, Hui, Yang, Xu: "The ROS/JNK/ATF2 pathway mediates selenite-induced leukemia NB4 cell cycle arrest and apoptosis in vitro and in vivo." in: Cell death & disease, Vol. 4, pp. e973, (2013) (PubMed).

Target Details for CD33

Target: CD33

Alternative Name: CD33

Background:

Synonyms: p67, SIGLEC3, SIGLEC-3, Myeloid cell surface antigen CD33, Sialic acid-binding Ig-like lectin 3, gp67, CD33

Background: Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro).

Gene ID: 945

UniProt: P20138