NOS2 antibody (AA 14-100) (HRP)

Catalog No. ABIN677237

The Rabbit Polyclonal anti-NOS2 antibody has been validated for WB, ELISA, IHC (p) and IHC (fro). It is suitable to detect NOS2 in samples from Human, Mouse, Rat and Chicken.

Quick Overview for NOS2 antibody (AA 14-100) (HRP) (ABIN677237)

Target: NOS2 (Nitric Oxide Synthase 2, Inducible (NOS2))

Reactivity: Human, Mouse, Rat, Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NOS2 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-NOS2 Antibody (HRP)

Binding Specificity: AA 14-100

Cross-Reactivity: Chicken, Human, Mouse, Rat

Predicted Reactivity: Dog,Pig,Rabbit,Guinea Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from mouse NOS-2

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for NOS2

Target: NOS2 (Nitric Oxide Synthase 2, Inducible (NOS2))

Alternative Name: iNOS

Background:

Synonyms: iNOS, Nos-2, Nos2a, i-NOS, NOS-II, Nitric oxide synthase, inducible, Inducible NO synthase, Inducible NOS, Macrophage NOS, MAC-NOS, NOS type II, Peptidyl-cysteine S-nitrosylase NOS2, Nos2, Inosl

Background: Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2.

Gene ID: 18126

UniProt: P29477

Pathways: Retinoic Acid Receptor Signaling Pathway, Cellular Response to Molecule of Bacterial Origin, Inositol Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process