Heme Oxygenase 1, Heme Oxygenase 2 (AA 101-200) antibody (HRP)

Catalog No. ABIN677282

The Rabbit Polyclonal anti- antibody has been validated for WB, ELISA, IHC (fro) and IHC (p). It is suitable to detect in samples from Chicken, Human, Mouse, Pig and Rat.

Quick Overview for Heme Oxygenase 1, Heme Oxygenase 2 (AA 101-200) antibody (HRP) (ABIN677282)

Target: Heme Oxygenase 1, Heme Oxygenase 2

Reactivity: Chicken, Human, Mouse, Pig, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details

Binding Specificity: AA 101-200

Cross-Reactivity: Chicken, Human, Mouse, Pig, Rat

Predicted Reactivity: Dog,Cow,Sheep,Horse,Rabbit,Guinea Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Heme Oxygenase 1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Heme Oxygenase 1, Heme Oxygenase 2

Background:

Synonyms: HO-1, HSP32, HMOX1D, bK286B1, HMOX1, HO, HO1

Background: Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Exhibits cytoprotective effects since excess of free heme sensitizes cells to undergo apoptosis.

Gene ID: 3162

UniProt: P09601