DC-SIGN/CD209 antibody

Catalog No. ABIN6774197

This anti-DC-SIGN/CD209 antibody is a Mouse Monoclonal antibody detecting DC-SIGN/CD209 in WB, FACS, IHC and ELISA. Suitable for Human.

Quick Overview for DC-SIGN/CD209 antibody (ABIN6774197)

Target: DC-SIGN/CD209 (CD209) (CD209)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This DC-SIGN/CD209 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA

Product Details anti-DC-SIGN/CD209 Antibody

Specificity: Recognizes human DC-SIGN on transfected NIH/3T3 cells and on monocyte derived dendritic cells at 44kD. Does not react with parental mouse cells or irrelevant transfectants, such as human DC-SIGN2.

Purification: Protein G purified

Immunogen: NIH/3T3 transfected with recombinant, human DC-SIGN.

Isotype: IgG2b

Application Details

Application Notes: Approved: ELISA, Flo, IHC (25 μg/mL), WB (1 - 2 μg/mL)

Usage: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Adhesion Blockade and Flow Cytometry. Flow Cytometry (Immunophenotyping): 10 μL of 25-50 μg/mL labels up to 10^6 cells. Adhesion Blockade: 5 μg/mL (5x10^6 cells/well). Immunohistochemistry: 25 μg/mL detects human DC-SIGN in fixed cells and tissue sections. Western Blot: 1-2 μg/mL The detection limit is a ~5 ng/well under non-reducing and reducing conditions using a colorimetric detection system,. Chemiluminescent detection will increase sensitivity by 5 to 50 fold.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile 40-50 % glycerol, PBS.

Concentration: Lot specific

Buffer: Lyophilized from in PBS,  pH 7.2, 5 % trehalose

Storage: -20 °C

Storage Comment: Lyophilized powder may be stored at -20°C for short-term only. Stable for 1 year at -20°C. Aliquot and store at -20°C. Reconstituted product is stable for 1 year at -20°C.

Target Details for DC-SIGN/CD209

Target: DC-SIGN/CD209 (CD209) (CD209)

Alternative Name: CD209 / DC-SIGN

Background: Name/Gene ID: CD209

Synonyms: CD209, CD209 antigen, CD209 Molecule, CDSIGN, CLEC4L, DC-SIGN, HIV gpl20-binding protein, DC-SIGN1

Gene ID: 30835

UniProt: Q9NNX6