Integrin alpha 1 antibody (AA 1001-1179) (HRP)

Catalog No. ABIN677492

The Rabbit Polyclonal anti-Integrin alpha 1 antibody has been validated for WB, ELISA, IHC (fro) and IHC (p). It is suitable to detect Integrin alpha 1 in samples from Human and Rat.

Quick Overview for Integrin alpha 1 antibody (AA 1001-1179) (HRP) (ABIN677492)

Target: Integrin alpha 1 (ITGA1)

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Integrin alpha 1 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Integrin alpha 1 Antibody (HRP)

Binding Specificity: AA 1001-1179

Specificity: This antibody may also detect integrin alpha L due to a 75 % non-contiguous sequence similarity in the corresponding immunogen region.

Cross-Reactivity: Human, Rat

Predicted Reactivity: Mouse,Cow,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Integrin alpha 1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Integrin alpha 1

Target: Integrin alpha 1 (ITGA1)

Alternative Name: Integrin alpha 1

Background:

Synonyms: VLA1, CD49a, Integrin alpha-1, CD49 antigen-like family member A, Laminin and collagen receptor, VLA-1, ITGA1

Background: Integrin alpha-1/beta-1 is a receptor for laminin and collagen. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. Involved in anchorage-dependent, negative regulation of EGF-stimulated cell growth.

Gene ID: 3672

UniProt: P56199

Pathways: EGFR Signaling Pathway, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor, Integrin Complex