Integrin, alpha E2 (AA 1101-1179) antibody

Catalog No. ABIN678758

Product Details

Target: Integrin, alpha E2

Binding Specificity: AA 1101-1179

Reactivity: Rat, Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Dog,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Integrin alpha-E heavy chain

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details

Target: Integrin, alpha E2

Background:

Synonyms: Itgae, A530055J10, alpha-E1, alpha-M290, aM290, CD103, MGC183601, antigen CD103, human mucosal lymphocyte antigen 1, alpha polypeptide.ITAE_HUMAN, Integrin alpha-E, HML-1 antigen, Integrin alpha-IEL, Mucosal lymphocyte 1 antigen, Integrin alpha-E heavy chain.

Background: CD103/Integrin alpha E is a new member of the integrin series of adhesion molecules containing the beta7 subunit. This antigen defines a developmentally important subset of T cells, namely mucosal T cells including all IEL (intraepithelial lymphocytes) and ~20 % of lamina propria T cells. Expression of CD103 is more restricted outside these mucosal organs, appearing at lower levels on T cell subsets of the lymph node, dendritic epidermis and periphery. In non-epithelial CD103+ T cells there is a bias toward expression on CD8+ cells. Integrin alpha E is a heterodimer comprising a 150 kDa alpha chain and a 120 kDa beta chain. Integrin alpha E is expressed by approximately 1 % of peripheral blood lymphocytes, activated T lymphocytes and by hairy cell leukaemia cells. The antigen is also expressed by intraepithelial lymphocytes.

Molecular Weight: 128kDa

Gene ID: 3682