Endonuclease G antibody (AA 101-200) (HRP)

Catalog No. ABIN680902

This Rabbit Polyclonal antibody specifically detects Endonuclease G in WB, ELISA, IHC (p) and IHC (fro). It exhibits reactivity toward Rat and Mouse.

Quick Overview for Endonuclease G antibody (AA 101-200) (HRP) (ABIN680902)

Target: Endonuclease G (ENDOG)

Reactivity: Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Endonuclease G antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Endonuclease G Antibody (HRP)

Binding Specificity: AA 101-200

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Human,Dog,Cow,Sheep,Pig,Horse,Rabbit,Guinea Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from rat Endonuclease G

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Endonuclease G

Target: Endonuclease G (ENDOG)

Alternative Name: Endonuclease G

Background:

Synonyms: EndoG, Endonuclease G, Endonuclease G mitochondrial, EndonucleaseG, FLJ27463, ENDOG, NUCG_HUMAN, Endo G.

Background: The protein encoded by this gene is a nuclear encoded endonuclease that is localized in the mitochondrion. The encoded protein is widely distributed among animals and cleaves DNA at GC tracts. This protein is capable of generating the RNA primers required by DNA polymerase gamma to initiate replication of mitochondrial DNA. [provided by RefSeq, Jul 2008].

Gene ID: 362100

Pathways: Apoptosis