Vitamin D Receptor antibody (AA 65-180)

Catalog No. ABIN682513

This Rabbit Polyclonal antibody specifically detects Vitamin D Receptor in WB, IF (cc), IF (p), IHC (p) and IHC (fro). It exhibits reactivity toward Human, Mouse and Rat and has been mentioned in 5+ publications.

Quick Overview for Vitamin D Receptor antibody (AA 65-180) (ABIN682513)

Target: Vitamin D Receptor (VDR)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Vitamin D Receptor antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Vitamin D Receptor Antibody

Binding Specificity: AA 65-180

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Cow,Pig,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Vitamin D Receptor

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-Vitamin D Receptor antibody (ABIN682513)

Wu, Chen, Tian, Zhang, Bian, Cheng: "Serum 25-hydroxyvitamin D inversely associated with blood eosinophils in patients with persistent allergic rhinitis." in: Asia Pacific allergy, Vol. 7, Issue 4, pp. 213-220, (2017) (PubMed).

Zhang, Zhou, Guo, Song, Liu: "1,25-Dihydroxyvitamin D₃ Promotes High Glucose-Induced M1 Macrophage Switching to M2 via the VDR-PPARγ Signaling Pathway." in: BioMed research international, Vol. 2015, pp. 157834, (2016) (PubMed).

Tian, Lv, Yang, Zhang, Yu, Zhu, Xiao, Zhu: "Effects of vitamin D on renal fibrosis in diabetic nephropathy model rats." in: International journal of clinical and experimental pathology, Vol. 7, Issue 6, pp. 3028-37, (2014) (PubMed).

Song, Guo, Zhou, Zhang: "The PI3K/p-Akt signaling pathway participates in calcitriol ameliorating podocyte injury in DN rats." in: Metabolism: clinical and experimental, Vol. 63, Issue 10, pp. 1324-33, (2014) (PubMed).

Zhang, Song, Guo, Zhou: "The novel role of TRPC6 in vitamin D ameliorating podocyte injury in STZ-induced diabetic rats." in: Molecular and cellular biochemistry, (2014) (PubMed).

Target Details for Vitamin D Receptor

Target: Vitamin D Receptor (VDR)

Alternative Name: Vitamin D Receptor/VDR

Target Type: Chemical

Background:

Synonyms: NR1I1, PPP1R163, Vitamin D3 receptor, VDR, 1,25-dihydroxyvitamin D3 receptor, Nuclear receptor subfamily 1 group I member 1

Background: Nuclear hormone receptor. Transcription factor that mediates the action of vitamin D3 by controlling the expression of hormone sensitive genes. Regulates transcription of hormone sensitive genes via its association with the WINAC complex, a chromatin-remodeling complex. Recruited to promoters via its interaction with the WINAC complex subunit BAZ1B/WSTF, which mediates the interaction with acetylated histones, an essential step for VDR-promoter association. Plays a central role in calcium homeostasis.

Gene ID: 7421

UniProt: P11473

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway