MFN2 antibody (AA 1-120)

Catalog No. ABIN682528

This Rabbit Polyclonal antibody specifically detects MFN2 in WB, ELISA, FACS, IHC (p), IF (cc), IF (p) and IHC (fro). It exhibits reactivity toward Human, Rat, Mouse and Rabbit and has been mentioned in 2+ publications.

Quick Overview for MFN2 antibody (AA 1-120) (ABIN682528)

Target: MFN2 (Mitofusin 2 (MFN2))

Reactivity: Human, Rat, Mouse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MFN2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-MFN2 Antibody

Binding Specificity: AA 1-120

Cross-Reactivity: Human, Mouse, Rabbit, Rat

Predicted Reactivity: Dog,Cow,Pig,Horse,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Mitofusin 2

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-MFN2 antibody (ABIN682528)

Hui, Lu, Han, Zhou, Liu, Li, Jin: "Resveratrol improves mitochondrial function in the remnant kidney from 5/6 nephrectomized rats." in: Acta histochemica, Vol. 119, Issue 4, pp. 392-399, (2017) (PubMed).

Ku, Ji, Zhang, Li, Sang: "PM2.5, SO2 and NO2 co-exposure impairs neurobehavior and induces mitochondrial injuries in the mouse brain." in: Chemosphere, Vol. 163, pp. 27-34, (2016) (PubMed).

Target Details for MFN2

Target: MFN2 (Mitofusin 2 (MFN2))

Alternative Name: Mitofusin 2

Background:

Synonyms: HSG, MARF, CMT2A, CPRP1, CMT2A2, Mitofusin-2, Transmembrane GTPase MFN2, MFN2, KIAA0214

Background: Essential transmembrane GTPase, which mediates mitochondrial fusion. Fusion of mitochondria occurs in many cell types and constitutes an important step in mitochondria morphology, which is balanced between fusion and fission. MFN2 acts independently of the cytoskeleton. It therefore plays a central role in mitochondrial metabolism and may be associated with obesity and/or apoptosis processes. Overexpression induces the formation of mitochondrial networks. Plays an important role in the regulation of vascular smooth muscle cell proliferation. Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). Is required for PARK2 recruitment to dysfunctional mitochondria. Involved in the control of unfolded protein response (UPR) upon ER stress including activation of apoptosis and autophagy during ER stress. Acts as an upstream regulator of EIF2AK3 and suppresses EIF2AK3 activation under basal conditions.

Gene ID: 9927

UniProt: O95140

Pathways: Skeletal Muscle Fiber Development