BCR antibody (pTyr177)

Catalog No. ABIN683653

This Rabbit Polyclonal antibody specifically detects BCR in WB, ELISA, FACS, IF (cc), IF (p), IHC (p) and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for BCR antibody (pTyr177) (ABIN683653)

Target: BCR (Breakpoint Cluster Region (BCR))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BCR antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-BCR Antibody

Binding Specificity: pTyr177

Cross-Reactivity: Human

Predicted Reactivity: Mouse,Rat,Pig,Rabbit,Guinea Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human Bcr around the phosphorylation site of Tyr177 [PF(p-Y)VN]

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for BCR

Target: BCR (Breakpoint Cluster Region (BCR))

Alternative Name: Bcr

Background:

Synonyms: Bcr phospho Y177, BcrTyr177, ALL, BCR 1, BCR/ABL FUSION GENE INCLUDED, BCR/FGFR1 FUSION GENE INCLUDED, BCR/PDGFRA FUSION GENE INCLUDED, BCR1, breakpoint cluster region, CML, PHL, BCR_HUMAN.

Background: The breakpoint cluster region protein (Bcr) is best know to be involved in genomic translocation with fusion partner Abl (Cbr-Abl) causing chronic myelogenous leukemia (CML). This 160 kDa protein contains a serine/threonine kinase domain, an SH2 binding domain, a GTP/GDP exchange domain and a C-term domain which functions as a GTPase activating protein for p21rac and CDC42. Additionally, Bcr is involved in signal transduction and can down regulate Ras mediated cell signaling.

Gene ID: 613

Pathways: Regulation of Leukocyte Mediated Immunity, Platelet-derived growth Factor Receptor Signaling