Cyclin B1 antibody (pSer147)

Catalog No. ABIN684448

This Rabbit Polyclonal antibody specifically detects Cyclin B1 in WB, ELISA, FACS, IHC (p), IF (cc), IF (p) and IHC (fro). It exhibits reactivity toward Humanand has been mentioned in 1 publication.

Quick Overview for Cyclin B1 antibody (pSer147) (ABIN684448)

Target: Cyclin B1 (CCNB1)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cyclin B1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Cyclin B1 Antibody

Binding Specificity: pSer147

Cross-Reactivity: Human

Predicted Reactivity: Mouse,Rat,Dog,Cow,Pig,Horse,Chicken,Rabbit,Guinea Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human Cyclin B1 around the phosphorylation site of Ser147

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-Cyclin B1 antibody (ABIN684448)

Hawash, Kahraman, Eren, Cetin Atalay, Baytas: "Synthesis and biological evaluation of novel pyrazolic chalcone derivatives as novel hepatocellular carcinoma therapeutics." in: European journal of medicinal chemistry, Vol. 129, pp. 12-26, (2017) (PubMed).

Target Details for Cyclin B1

Target: Cyclin B1 (CCNB1)

Alternative Name: Cyclin B1

Background:

Synonyms: Cyclin B1 phospho S147, Cyclin B1 Ser147, p-Cyclin B1 S147, p-Cyclin B1 Ser147, p-Cyclin B1 phospho S147, CCNB 1, CCNB, CCNB1, CCNB1_HUMAN, G2 mitotic specic cyclin B1, G2/mitotic-specic cyclin-B1.

Background: The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq, Jul 2008].

Gene ID: 891

Pathways: Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase