GCN2 antibody (pThr899)

Catalog No. ABIN684943

This Rabbit Polyclonal antibody specifically detects GCN2 in WB, ELISA and FACS. It exhibits reactivity toward Humanand has been mentioned in 1 publication.

Quick Overview for GCN2 antibody (pThr899) (ABIN684943)

Target: GCN2 (EIF2AK4) (Eukaryotic Translation Initiation Factor 2 alpha Kinase 4 (EIF2AK4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GCN2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-GCN2 Antibody

Binding Specificity: pThr899

Specificity: This phosphorylation site is homologous to Thr898 in Mouse and Thr899 in Rat.

Cross-Reactivity: Human

Predicted Reactivity: Mouse,Rat,Dog,Cow,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human GCN2 around the phosphorylation site of Thr899

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-GCN2 antibody (ABIN684943)

Lehman, Ryeom, Koumenis: "Signaling through alternative Integrated Stress Response pathways compensates for GCN2 loss in a mouse model of soft tissue sarcoma." in: Scientific reports, Vol. 5, pp. 11781, (2015) (PubMed).

Target Details for GCN2

Target: GCN2 (EIF2AK4) (Eukaryotic Translation Initiation Factor 2 alpha Kinase 4 (EIF2AK4))

Alternative Name: GCN2

Background:

Synonyms: GCN2, PVOD2, Eukaryotic translation initiation factor 2-alpha kinase 4, GCN2-like protein, EIF2AK4, KIAA1338

Background: GCN2 belongs to a family of kinases that phosphorylate the alpha subunit of eukaryotic translation initiation factor 2 to downregulate protein synthesis in response to varied cellular stresses.

Gene ID: 440275

UniProt: Q9P2K8

Pathways: ER-Nucleus Signaling, Hepatitis C