HIST1H1E antibody (pThr18) (HRP)

Catalog No. ABIN685192

This Rabbit Polyclonal antibody specifically detects HIST1H1E in ELISA, WB, IHC (p) and IHC (fro). It exhibits reactivity toward Mouse and Rat.

Quick Overview for HIST1H1E antibody (pThr18) (HRP) (ABIN685192)

Target: HIST1H1E (Histone H1.4 (HIST1H1E))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HIST1H1E antibody is conjugated to HRP

Application: ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-HIST1H1E Antibody (HRP)

Binding Specificity: pThr18

Specificity: This phosphorylation site is found in Rat H1C as well, but not Human or Mouse H1C. This phosphorylation site is homologous to Thr18 in Rat H1C/H1D and Mouse H1D. Due to the highly conserved nature of these histones, this antibody may react with these members when phosphorylated at this site.

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Human

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human Histone H1.4 around the phosphorylation site of Thr18

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for HIST1H1E

Target: HIST1H1E (Histone H1.4 (HIST1H1E))

Alternative Name: Histone H1.4

Background:

Synonyms: H1E, H1.4, H1F4, H1s-4, dJ221C16.5, Histone H1.4, Histone H1b, Histone H1s-4, HIST1H1E

Background: Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity).

Gene ID: 3008

UniProt: P10412