PRKDC antibody (AA 150-200) (Cy5.5)

Catalog No. ABIN685422

This anti-PRKDC antibody is a Rabbit Polyclonal antibody detecting PRKDC in WB. Suitable for Human, Mouse and Rat.

Quick Overview for PRKDC antibody (AA 150-200) (Cy5.5) (ABIN685422)

Target: PRKDC (Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRKDC antibody is conjugated to Cy5.5

Application: Western Blotting (WB)

Product Details anti-PRKDC Antibody (Cy5.5)

Binding Specificity: AA 150-200

Cross-Reactivity: Human, Rat

Predicted Reactivity: Mouse,Dog,Cow,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human DNA PKcs

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for PRKDC

Target: PRKDC (Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC))

Alternative Name: Dnapk/Prkdc

Background:

Synonyms: HYRC, p350, DNAPK, DNPK1, HYRC1, IMD26, XRCC7, DNA-PKcs, DNA-dependent protein kinase catalytic subunit, DNA-PK catalytic subunit, p460, PRKDC

Background: Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Required to protect and align broken ends of DNA. May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. Also involved in modulation of transcription. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX, thereby regulating DNA damage response mechanism. Phosphorylates DCLRE1C, c-Abl/ABL1, histone H1, HSPCA, c-jun/JUN, p53/TP53, PARP1, POU2F1, DHX9, SRF, XRCC1, XRCC1, XRCC4, XRCC5, XRCC6, WRN, MYC and RFA2. Can phosphorylate C1D not only in the presence of linear DNA but also in the presence of supercoiled DNA. Ability to phosphorylate p53/TP53 in the presence of supercoiled DNA is dependent on C1D. Contributes to the determination of the circadian period length by antagonizing phosphorylation of CRY1 'Ser-588' and increasing CRY1 protein stability, most likely through an indirect machanism. Interacts with CRY1 and CRY2, negatively regulates CRY1 phosphorylation.

Gene ID: 5591

UniProt: P78527

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response