Claudin 1 antibody (AA 121-211)

Catalog No. ABIN686407

This Rabbit Polyclonal antibody specifically detects Claudin 1 in WB, ELISA, IHC (p), IF (p), FACS, IF (cc) and IHC (fro). It exhibits reactivity toward Human, Rat and Mouseand has been mentioned in 1 publication.

Quick Overview for Claudin 1 antibody (AA 121-211) (ABIN686407)

Target: Claudin 1 (CLDN1)

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Claudin 1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Claudin 1 Antibody

Binding Specificity: AA 121-211

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from mouse Claudin 1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-Claudin 1 antibody (ABIN686407)

Wang, Chen, Liang, Yan, Lin, Liu, Luo, Huang, Li, Liu, Tang: "Notch inhibition promotes fetal liver stem/progenitor cells differentiation into hepatocytes via the inhibition of HNF-1?." in: Cell and tissue research, Vol. 357, Issue 1, pp. 173-84, (2014) (PubMed).

Target Details for Claudin 1

Target: Claudin 1 (CLDN1)

Alternative Name: Claudin 1

Background:

Synonyms: AI596271, Claudin-1, Cldn1

Background: Claudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia. While some claudin family members play essential roles in the formation of impermeable barriers, others mediate the permeability to ions and small molecules. Often, several claudin family members are coexpressed and interact with each other, and this determines the overall permeability. CLDN1 is required to prevent the paracellular diffusion of small molecules through tight junctions in the epidermis and is required for the normal barrier function of the skin. Required for normal water homeostasis and to prevent excessive water loss through the skin, probably via an indirect effect on the expression levels of other proteins, since CLDN1 itself seems to be dispensable for water barrier formation in keratinocyte tight junctions.

Gene ID: 12737

UniProt: O88551

Pathways: Cell-Cell Junction Organization, Hepatitis C