GAPDH antibody

Catalog No. ABIN6923148

This anti-GAPDH antibody is a Mouse Monoclonal antibody detecting GAPDH in WB, IHC, IF and ICC. Suitable for Human, Mouse, Rat, Pig, Cow and Mammalian.

Quick Overview for GAPDH antibody (ABIN6923148)

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Reactivity: Human, Mouse, Rat, Pig, Cow, Mammalian

Host: Mouse

Clonality: Monoclonal

Conjugate: This GAPDH antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: 1D4

Product Details anti-GAPDH Antibody

Purification: conc. tissue culture supernatant

Isotype: IgM

Application Details

Application Notes: The antibody solution can be used at dilutions of at least 1:100 in immunofluorescence experiments. In western blotting using chemiluminescence it can be used at dilutions of 1:1,000 or lower. It is very useful as a loading control for quantitative western blotting.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Supplied as purified antibody at 1mg/mL in PBS and glycerol (1:1) with 5mM NaN3

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4°C short term or -20°C long term.

Target Details for GAPDH

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Alternative Name: Glyceraldehyde 3-Phosphate Dehydrogenase

Background: Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) is a metabolic enzyme responsible for catalyzing one step in the glycolytic pathway, the reversible oxidative phosphorylation of glyceraldehyde 3-phosphate. Because GAPDH as a protein expressed in large amounts and which is required at all times for an important house keeping functions, levels of GAPDH mRNA are often measured and used as standards in studies of mRNA expression. Increasingly, scientists are making use of specific antibodies to GAPDH in comparable studies of levels of protein expression. This antibody can be used as a loading control for western blotting experiments, allowing comparison between the level of this protein and others in a cell or tissue. Apart from a role in glycolysis, GAPDH may have other roles such as in the activation of transcription. GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimer's disease, and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntington's disease. Associations with actin and tubulin have also been reported. The protein may also have a role in the regulation of apoptosis, and interestingly migrates from the cytoplasm into the nucleus when cells become apoptotic. The immunogen used to raise this particular antibody was extensively purified pig GAPDH. The HGNC name for this protein is GAPDH.