SNCA antibody (pSer129) (Atto 594)

Catalog No. ABIN6932864

This anti-SNCA antibody is a Rabbit Monoclonal antibody detecting SNCA in WB, ELISA, IHC and ICC. Suitable for Human.

Quick Overview for SNCA antibody (pSer129) (Atto 594) (ABIN6932864)

Target: SNCA (Synuclein, alpha (SNCA))

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This SNCA antibody is conjugated to Atto 594

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Clone: J18

Product Details anti-SNCA Antibody (Atto 594)

Binding Specificity: AA 124-134, pSer129

Specificity: Expressed principally in brain but is also expressed in low concentrations in all tissues examined except in liver. Concentrated in presynaptic nerve terminals.,Binds to phosphorylated serine 129 on alpha synuclein. Does not detect unphosphorylated serine 129 alpha synuclein

Cross-Reactivity: Human, Mouse

Purification: Affinity Purified

Immunogen: Human alpha synuclein AA 124-134: AYEMP-pS-EEGYQ-Cys

Isotype: IgG

Application Details

Application Notes:

• WB (1:500)
• optimal dilutions for assays should be determined by the user.

Comment:

A 1:500 dilution of ABIN6932864 was sufficient for detection of Alpha Synuclein pSer129 in 10 μg of Mouse Brain by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % Sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for SNCA

Target: SNCA (Synuclein, alpha (SNCA))

Alternative Name: Alpha Synuclein

Background: Alpha-Synuclein (SNCA) is expressed predominantly in the brain, where it is concentrated in presynaptic nerve terminals (1). Alpha-synuclein is highly expressed in the mitochondria of the olfactory bulb, hippocampus, striatum and thalamus (2). Functionally, it has been shown to significantly interact with tubulin (3), and may serve as a potential microtubule-associated protein. It has also been found to be essential for normal development of the cognitive functions, inactivation may lead to impaired spatial learning and working memory (4). SNCA fibrillar aggregates represent the major non A-beta component of Alzheimers disease amyloid plaque, and a major component of Lewy body inclusions, and Parkinson's disease. Parkinson's disease (PD) is a common neurodegenerative disorder characterized by the progressive accumulation in selected neurons of protein inclusions containing alpha-synuclein and ubiquitin (5, 6). Alpha synuclein phosphorylated at serine 129 constitutes 90 % of the alpha synuclein found in Lewy bodies (7, 8).

Gene ID: 6622

NCBI Accession: NP_000336

UniProt: P37840

Pathways: Synaptic Membrane, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Platelet-derived growth Factor Receptor Signaling, Negative Regulation of Transporter Activity, Regulation of long-term Neuronal Synaptic Plasticity