VPS35 antibody (Biotin)

Catalog No. ABIN6932920

The Mouse Monoclonal anti-VPS35 antibody has been validated for WB, IP, IF and ICC. It is suitable to detect VPS35 in samples from Human.

Quick Overview for VPS35 antibody (Biotin) (ABIN6932920)

Target: VPS35 (Vacuolar Protein Sorting 35 (VPS35))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This VPS35 antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: 7E4

Product Details anti-VPS35 Antibody (Biotin)

Specificity: Ubiquitous. Highly expressed in brain, colon, heart, kidney, ovary, placenta, skeletal muscle, small intestine, testis, thymus.,Detects ~92 kDa.

Cross-Reactivity: Human, Mouse, Rat

Purification: Protein G Purified

Immunogen: Full length recombinant human VSP35

Isotype: IgG2a

Application Details

Application Notes:

• WB (1:1000)
• ICC/IF (1:200)
• IP (1:200)
• optimal dilutions for assays should be determined by the user.

Comment:

A 1:1000 dilution of ABIN6932920 was sufficient for detection of VPS35 in 10 μg of SH-SY5Y by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % Sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for VPS35

Target: VPS35 (Vacuolar Protein Sorting 35 (VPS35))

Alternative Name: VPS35

Background: Vacuolar Protein Sorter-35 (VPS35) is a component of the retromer complex, which is essential for endosome-to-Golgi retrieval of membrane proteins. VPS35 mutations such as D620N have been linked to Parkinson's Disease (PD) (1,2) and affect retromer function, protein homeostasis, and mitochondria (3).

Gene ID: 55737

NCBI Accession: NP_060676

UniProt: Q96QK1