KRT77, KRT76 antibody (FITC)

Catalog No. ABIN6933729

Product Details

Target: KRT77, KRT76

Reactivity: Human, Rat, Chicken, Cow, Dog, Monkey, Mouse, Rabbit

Host: Mouse

Clonality: Monoclonal

Conjugate: FITC

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Test (T)

Purification: Purified by Protein A/G

Immunogen: Human epidermal keratin

Clone: AE-1-AE-3

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: Skin, Adeno- or Squamous carcinomas.

Known Application: Flow Cytometry: 5 µL per test per one million cells (or 5 µL per 100 µL of whole blood)Immunofluorescence (1:50-1:100)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 100 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA and 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 4 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details

Target: KRT77, KRT76

Alternative Name: KRT77 & KRT76

Background: Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 40 kDa (CK19). Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE-1/AE-3 is a broad spectrum anti pan-cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It has been used to characterize the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal and abnormal human tissues and has shown high sensitivity in the recognition of epithelial cells and carcinomas.

Molecular Weight: 40-67kDa

Gene ID: 374454, 51350

UniProt: Q7Z794, Q01546