CD28 antibody (CF®488A)

Catalog No. ABIN6934044

The Mouse Monoclonal anti-CD28 antibody has been validated for FACS, IF and T. It is suitable to detect CD28 in samples from Human.

Quick Overview for CD28 antibody (CF®488A) (ABIN6934044)

Target: CD28

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD28 antibody is conjugated to CF®488A

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Test (T)

Clone: CB28

Product Details anti-CD28 Antibody (CF®488A)

Specificity: Recognizes a glycoprotein of 44-88 kDa, which is identified as CD28. It is the critical T-cell co-stimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its co-stimulation role, CD28 functions in preventing T-cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.

Purification: Purified by Protein A/G

Immunogen: Recombinant human CD28 protein

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: Jurkat cells. Human lymph nodes and tonsils.

Known Application: Flow Cytometry: 5 µL per test per one million cells (or 5 µL per 100 µL of whole blood)Immunofluorescence (1:50-1:100)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 100 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA and 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 4 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for CD28

Target: CD28

Alternative Name: CD28

Molecular Weight: 44kDa (monomer), 88kDa (dimer)

Gene ID: 940

UniProt: P10747

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response