EpCAM antibody (N-Term) (CF®488A)

Catalog No. ABIN6934075

This anti-EpCAM antibody is a Mouse Monoclonal antibody detecting EpCAM in FACS, IF, IHC and T. Suitable for Human.

Quick Overview for EpCAM antibody (N-Term) (CF®488A) (ABIN6934075)

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This EpCAM antibody is conjugated to CF®488A

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Test (T)

Clone: EGP40-826

Product Details anti-EpCAM Antibody (CF®488A)

Binding Specificity: AA 20-60, N-Term

Specificity: Recognizes a 40-43 kDa transmembrane epithelial glycoprotein, identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). Ep-CAM is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. It is also useful in distinguishing serous carcinomas of the ovary from mesothelioma. This epithelial antigen plays an important role as a tumor-cell marker in lymph nodes from patients with esophageal carcinoma otherwise classified as node-negative. Epithelial antigen has also been suggested as a discriminator between basal cell and baso-squamous carcinomas, and squamous cell carcinoma of the skin.

No Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Purification: Purified by Protein A/G

Immunogen: A synthetic peptide (around aa 20-60) from the N-terminus of human TACSTD1 protein

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: HT29 or SK-OV-3 cells (FACS/IF). Breast carcinoma (IHC).

Known Application: Flow Cytometry (5 µL per test per one million cells or 5 µL per 100 µL of whole blood),Immunofluorescence (1:50-1:100),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 100 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 4 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for EpCAM

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Alternative Name: TACSTD1

Molecular Weight: 40-43kDa

Gene ID: 4072

UniProt: P16422