S100B antibody (CF®488A)

Catalog No. ABIN6934620

The Mouse Monoclonal anti-S100B antibody has been validated for IF, FACS and T. It is suitable to detect S100B in samples from Human, Rat, Mouse and Cow.

Quick Overview for S100B antibody (CF®488A) (ABIN6934620)

Target: S100B (S100 Calcium Binding Protein B (S100B))

Reactivity: Human, Rat, Mouse, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This S100B antibody is conjugated to CF®488A

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Test (T)

Clone: S100B-1012

Product Details anti-S100B Antibody (CF®488A)

Specificity: S100 belongs to the family of calcium binding proteins. S100A and S100B proteins are two members of the S100 family. S100A is composed of an alpha and a beta chain whereas S100B is composed of two beta chains. This antibody is specific against an epitope located on the beta-chain (i.e. in S-100A and S-100B) but not on the alpha-chain of S-100 (i.e. in S-100A and S100A0). This antibody can be used to localize S-100A and S-100B in various tissue sections. S-100 protein has been found in normal melanocytes, Langerhans cells, histiocytes, chondrocytes, lipocytes, skeletal and cardiac muscle, Schwann cells, epithelial and myoepithelial cells of the breast, salivary and sweat glands, as well as in glial cells. Neoplasms derived from these cells also express S-100 protein, albeit non-uniformly. A large number of well-differentiated tumors of the salivary gland, adipose and cartilaginous tissue, and Schwann cell-derived tumors express S-100 protein. Almost all malignant melanomas and cases of histiocytosis X are positive for S-100 protein.

Purification: Purified by Protein A/G

Immunogen: Recombinant full-length human S100B protein

Isotype: IgG2a kappa

Application Details

Application Notes:

Positive Control: Brain, Melanoma.

Known Application: Flow Cytometry (5 µL per test per one million cells or 5 µL per 100 µL of whole blood),Immunofluorescence (1:50-1:100),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 100 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA and 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 4 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for S100B

Target: S100B (S100 Calcium Binding Protein B (S100B))

Alternative Name: S100B

Molecular Weight: 10-12kDa

Gene ID: 6285

UniProt: P04271

Pathways: Regulation of Muscle Cell Differentiation, Positive Regulation of Immune Effector Process, Toll-Like Receptors Cascades, Regulation of long-term Neuronal Synaptic Plasticity, S100 Proteins