CD33 antibody (PE)

Catalog No. ABIN6935505

The Mouse Monoclonal anti-CD33 antibody has been validated for FACS, IF and T. It is suitable to detect CD33 in samples from Human.

Quick Overview for CD33 antibody (PE) (ABIN6935505)

Target: CD33

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD33 antibody is conjugated to PE

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Test (T)

Clone: WM53

Product Details anti-CD33 Antibody (PE)

Specificity: Recognizes a 67 kDa glycoprotein, which is identified as CD33 (HLDA IV, WS Code M-505). CD33 is a transmembrane protein of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. It belongs to the immunoreceptor tyrosine-based inhibitory motif (ITIM)-containing molecules able of recruiting protein tyrosine phosphatases SHP-1 and SHP-2 to signal assemblies, these ITIMs are also used for ubiquitin-mediated removal of the receptor from the cell surface. CD33 is expressed on cells of myelomonocytic lineage, binds sialic acid residues in N- and O-glycans on cell surfaces, and is a therapeutic target for acute myeloid leukemia. CD33 is expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells. It is absent on platelets, lymphocytes, erythrocytes and hematopoietic stem cells.

Purification: Purified by Protein A/G

Immunogen: Human AML cells

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: Human dendritic and mast cells. Human lymph nodes and tonsils.

Known Application: Flow Cytometry: 5 µL per test per one million cells (or 5 µL per 100 µL of whole blood)Immunofluorescence (1:50-1:100)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 100 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA and 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 4 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for CD33

Target: CD33

Alternative Name: CD33

Molecular Weight: 67kDa

Gene ID: 945

UniProt: P20138